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JSRM Code: 014020700014      Full Text PDF

Proceedings of Journal of Stem Cells and Regenerative Medicine (JSRM) Oration-2018

Germ-line stem cells in myocardial regeneration: Secretion of cardiogenic paracrine effectors may be the future
Gallicano I1

Author Names in full:G. Ian Gallicano

1Department of Biochemistry and Molecular & Cell Biology, Georgetown University Medical Center, Washington, DC, USA

Citation: Gallicano I. Germ-line stem cells in myocardial regeneration: Secretion of cardiogenic paracrine effectors may be the future. Proceedings of Journal of Stem Cells and Regenerative Medicine (JSRM) Oration-2018; J Stem Cells Regen Med 2018; 14(2) : 87

Stem cell research for treating or curing ischemic heart disease has, to date, culminated in identifying which scenario is more important; 1) stem cell differentiation into cardiomyocytes that integrate electrically with the heart, 2) stem cells that secrete paracrine factors that promote healing, or 3) a combination of both.  We consistently found that unipotent germline stem cells, when removed from their niche and cultured in the correct medium endogenously express pluripotency genes, which induce them to become human germline pluripotent stem cells (hgPSCs). These cells are then capable of producing cell types from all three germ layers. Using hgPSCs along with a modified version of a relatively novel cell-expansion culture methodology to induce quick, indefinite expansion of normally slow growing hgPSCs, it was possible to test the potential of cardiomyocytes derived from hgPSCs for treating an ischemic cardiac event. Upon differentiation into cardiac lineages, our data consistently showed that they not only express cardiac genes, but also express cardiac-promoting paracrine factors. Taking these data a step further, we found that hgPSC-derived cardiac cells can integrate into cardiac tissue in vivo. Note, while the work presented here was based on testes-derived hgPSCs, data from other laboratories have shown that ovaries contain very similar types of stem cells that can give rise to hgPSCs. As a result, hgPSCs should be considered a viable option for eventual use in patients, male or female, with ischemic heart disease.

Sponsors

The Journal of Stem Cells and Regenerative Medicine (JSRM) Oration-2018 was jointly sponsored by German Society for Stem Cell Research (GSZ)

This lecture was based on the Article "Re-Defining Stem Cell-Cardiomyocyte Interactions: Focusing on the Paracrine Effector Approach"; published in JSRM 2018 Vol. 14, Iss. 1 - Chosen as the "Article of the year 2018" by the Editorial Board of JSRM. The lecture was delivered in the JSRM Oration 2018 session of NCRM NICHE (www.ncrmniche.org) which is a day of commemorative events to celebrate the inaugural Anniversary of Nichi-In Centre for Regenerative Medicine, conducted every year since 2006 in the month of October. NCRM NICHE provides a platform that augments the interaction of scientists and clinicians. It enables them to exchange ideas in order to arrive at synergies while working towards a common goal of discovering clinically applicable solutions for diseases without a definitive treatment to yield relief to numerous patients. Conducted in India till 2016, it has been conducted in Japan from 2017 onwards. NCRM NICHE has two components: 1. Active Knowledge Gaining (AKG) events which refer to any knowledge gaining activity in which the participant plays an active role before and during the event instead of being a passive listener to a lecture by a speaker or a conversation among third parties. AKG events of NCRM NICHE include a. Fujio Cup Quiz (FCQ); b. Oral Presentation session and c. Tour to Premier Institutes and Inter Disciplinary Conclave (IDC); 2. Passive Knowledge Gaining (PKG) events which refer to an event, where there is no mandatory preparation by the participants before the event. During the event, they have to merely be a passive listener to others either delivering a lecture or interacting among third parties. PKG events of NCRM NICHE include a. Lectures & Orations and b. Inter-Disciplinary Interactions and Solutions (IIDIAS) Session. NCRM NICHE 2018 was conducted at the Shibaura Institute of Technology, Tokyo, Japan from 21 ~ 23 October 2018.