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Vol.IV Issue: I: Abstract: PASRM 2008-008 (JSRM code: 004010700010) | ![]() |
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Envisaging an allogenic Corneal endothelial precursor/Stem Cell Bank (CESBANK) Parikumar P1, Nelson J2, John S2, Baskar S3, Senthil KR3, Murugan P3, Senthilnagarajan R3, Srinivasan V3, Abraham S3,4, Amano S5 (PASRM** 2008-008) 1. The Light Eye Hospital, Dharmapuri, India. 2. Joseph Eye Hospital, Trichy, India. 3. Nichi-In Centre for Regenerative Medicine, Chennai, India. 4. Yamanashi University - Faculty of Medicine, Chuo, Japan. 5. Tokyo University-School of Medicine, Tokyo, Japan |
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Background: Bullous Keratopathy (BK) affects thousands of people in India every year. Though in early stages it is manageable medically, advanced disease warrants either total corneal transplantation or partial thickness transplantation for which a donor-cadaver cornea is necessary. Amano et al have reported the successful treatment of BK in animal models using in-vitro expanded human corneal endothelial precursors; though the rabbits had to be kept facing eye down to allow gravity assisted settling of the cells to the summit of the cornea where the damage had been created. For successful treatment using the above method, a human being has to lie prone with eyes immobilized for 24-36 Hrs. This is extremely discomforting and hence not practical. Corneal endothelium removed from the button and transported at varying temperature conditions for 48Hrs was successfully cultured in NCRM and this was reported earlier. We are working on a suitable scaffold to retain the cells in situ until their attachment to the damaged portion of the corneal endothelium enabling it to heal without the patient having to lie prone. With such capability, we envisage to make a corneal endothelial precursor/stem cell (CES) bank named as CESBANK to make in-vitro expanded CES available for patients with corneal diseases, most commonly Bullous Keratopathy (BK). Conclusion:@ The present project when implemented could make one donor eye be usable to more than 5 to 14 recipient eyes. By increasing the awareness for eye donation, more patients with corneal endothelial diseases awaiting donor cornea could be treated. Corresponding Author: Dr. P. Parikumar, The Light Eye Hospital, 39D, By-pass Road, Dharmapuri Lodge Compound, Dharmapuri, Dharmapuri Dist, India. Tel: +91 4342 268368. Email: pparikumar@yahoo.com . @ ** Proceedings of Annual Symposium on Regenerative Medicine |
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