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Mouse iPSC generated with porcine reprogramming factors as a model for studying the effects of non-silenced heterologous transgenes on pluripotency
Petkov SG1, Glage S2, Niemann H3

Author Names in full: Stoyan G Petkov1, Silke Glage2, Heiner Niemann3

1 German Primate Center, Goettingen, Germany, 2 Hannover Medical School, Hannover, Germany, 3 Institute for Farm Animal Genetics (FLI), Neustadt, Germany

 

Supplementary Figure


Supplementary Figure 1: Karyotypes of miPSC produced with porcine reprogramming factors. A) Metaphase spread of a TetO-miPSC line 2 at passage 59. B) Metaphase spread of CAG-miPSC line 1 at passage 60.

Supplementary Figure 2: BLAST alignments of amino acid sequences of mouse and porcine reprogramming factors (http://blast.ncbi.nlm.nih.gov). A) Alignment of Mus Musculus (NP_038661.2) and Sus Scrofa (NP_001106531) Oct4 amino acid sequences. B) Alignment of Mus Musculus (AAH57574.1) and Sus Scrofa (NP_001116669.1) Sox2 amino acid sequences . C) Alignment of Mus Musculus (AAB59728.1) and Sus Scrofa (CAA65753.1) c-myc amino acid sequences. D) Alignment of Mus Musculus (NP_034767.2) and Sus Scrofa (ACF93739.1) Klf4 amino acid sequences.

Supplementary Table

Table S1. Primers used for RT-PCR and real-time PCR

Supplementary Video